We recently noticed problems to map next generation sequencing reads to certain regions of the Streptococcus genome, our pet organism for the study of pathogenic bacteria at the MPUSP. An extensive re-sequencing effort using short and long read methods revealed that the original assembly contained various errors, particularly for repetitive rRNA/tRNA operons (published now in MRA). Our new assembly fixes these errors and adds two new tRNAs, becoming the current reference assembly for Streptococcus pyogenes. It’s available on NCBI datasets.